Optimizing Recombinant Cas9 Expression: Insights from E. coli BL21(DE3) Strains for Enhanced Protein Purification and Genome Editing

The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing.Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely.However, while CRISPR-Cas9 presents notable benefits and encouraging outcomes as a molecular tool and a potentia

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Complementarity in the development of rural tourism with the development of thermal baths, spa and wellness tourism

Serbia has a long tradition in thermal baths tourism development.In the second half of the twentieth century, this branch of tourism attracted a significant segment of tourist demand, both domestic and foreign.However, due to difficult business conditions in the nineties, its infrastructure became outdated.This resulted in negative trends in touris

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Fatigue Characteristics of Magnetostrictive Thin-Film Coated Surface Acoustic Wave Devices for Sensing Magnetic Field

Magnetostrictive thin-film coated Surface Acoustic Wave (SAW) devices were promising for sensing magnetic field owing to their superior features as micro-size, fast response, and high sensitivity originated from the magnetostrictive effect.However, the magnetostriction nature in magnetostrictive thin-film causes significantly mechanical fatigue in

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